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. 2013 Mar 25;168(8):1868–1877. doi: 10.1111/bph.12081

Figure 2.

Figure 2

Aortic ALDH2 mRNA (A) and protein (B) levels of nitrate-tolerant WT, ascorbate-supplemented (Asc-suppl.), ascorbate-deficient (Asc-def.) and bortezomib-treated ascorbate-deficient Gulo(-/-) aortas relative to untreated WT controls. mRNA levels were analysed according to the 2-ΔΔCt method using cyclophilin D as reference gene. ALDH2 protein expression was analysed by immunoblotting and quantified densitometrically with band intensities of samples from untreated WT mice (applied on the same gels) set to 100%. A representative blot is shown as inset. Data are mean values ± SEM of four (A) or 6–9 (B) animals. *P < 0.05 compared with untreated (non-tolerant) WT animals. #P < 0.05 compared with ascorbate-supplemented mice.