Fig 6.

eIF4G/-4A/-4B helicase complex formation and/or association with eIF3 and the 40S ribosomal subunit with full-length eIF4G or with HEAT-1. (A and B) Dox-inducible HEK293 cells expressing myc-eIF4G(197-1600)-Flag (A) or HEK293 cells transfected (16 h) with myc-eIF4G(682-1132)-Flag (B) were serum starved (24 h) and treated for the intervals shown with DMSO or 200 nM TPA. Lysates were subjected to Flag-IP/immunoblotting with the indicated antibodies. The experiments were repeated three times, and the results of representative assays are shown.