Fig 7.

Fatty acid profiling of WT and mutant yeast strains. (A) Longer-chain fatty acid-containing sphingolipid species. IPC species with 44 and 46 carbon-containing acyl chains are shown. The VLCFA and ceramide mutants sur4Δ and lip1Δ synthase are included as controls. (B) Shorter-chain fatty acid-containing sphingolipid species. Three IPC species with 38, 40, and 42 carbon-containing acyl chains are shown. For both panels A and B, the data represent the percentage of signal of each lipid species normalized to the signals of the PI 16:0-18:1 and PI 16:1-18:0 ions.