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eIF2B localization in the ybr159wΔ background. (A) Live cell fluorescence microscopy of endogenously tagged eIF2B subunit GCD7-GFP. Bright-field (BF) images are included for clarity. (B) Immunofluorescence microscopy of formaldehyde-fixed yeast cells. A polyclonal antibody against yeast eIF2B subunit GCD6 was used along with an Alexa Fluor 488-tagged secondary. Nuclei are stained with DAPI for clarity.
