Table 3.
Primers used in this study
| Primer | Orientationa | Sequence (5′–3′) |
|---|---|---|
| Genomic YBR159W deletion primers | ||
| YBR159W deletion primer | F | CGTACGCTGCAGGTCGAC |
| R | ATCGATGAATTCGAGCTCG | |
| 5′ Homology extension primer | F | CGGATTTGGAAGTCCTTTATAG |
| R | GTCGACCTGCAGCGTACGCATTTCTTAAGCTGCACCG | |
| 3′ Homology extension primer | F | CGAGCTCGAATTCATCGATTAGAATTATCGTTCTCG |
| R | GGACTTGGTCCTTCCACC | |
| Yeast two-hybrid primers | ||
| Two-hybrid common primer | F | CTATCTATTCGATGATGAAGATACCCCACCAAACCCAAAAAAAGAGATCGAATTCCAGCTGACCACCATG |
| R | GTACCGTTAAGGGCCCCTAGGCAGCTGGACGTCTCTAGATACTTAGCATCTATGACTTTTTGGGGCGTTC | |
| Two-hybrid YBR159W | F | AATTCCAGCTGACCACCATGACTTTTATGCAACAGCTTCAAGAGGCTGG |
| R | GATCCCCGGGAATTGCCATGCTATTCCTTTTTAACCTGTCTTGCGGCTTTTTTTAAGG | |
| Two-hybrid GCD1 | F | AATTCCAGCTGACCACCATGTCAATTCAGGCTTTTGTCTTTTGCGGTAAAGG |
| R | GATCCCCGGGAATTGCCATGTTAACGCTCAAATAATCCGTCATCTTCGTACTCGTAC | |
| Two-hybrid GCD2 | F | AATTCCAGCTGACCACCATGAGCGAATCGGAAGCCAAATCTAGGTCG |
| R | GATCCCCGGGAATTGCCATGTTATGCGGAACCTTTGTACTCTCTTAAAATAACAGGGAC | |
| Two-hybrid GCD6 | F | AATTCCAGCTGACCACCATGGCTGGAAAAAAGGGACAAAAGAAAAGTGGACTAG |
| R | GATCCCCGGGAATTGCCATGTTATTCCTCTTCTGAGGAAGATTCTTCGTCAGCATTC | |
| Two-hybrid GCD7 | F | AATTCCAGCTGACCACCATGTCCTCTCAAGCATTCACTTCAGTACATCCG |
| R | GATCCCCGGGAATTGCCATGTCACGCCTTATTTTTATCCAAATGCACATCAATTTGC | |
| YBR159W ORF + 600-bp upstream primers | ||
| 600-bp upstream YBR159W primer | F | CACCATGGTTTTTGTGACTTTACCTATAAATAGTACACAAC |
| YBR159W ORF primer | R | CTATTCCTTTTTAACCTGTCTTGCGGCTTTTTTTAAGGC |
| pAG415GAL-ccdB promoter remover 1 | GGGAGCTCCATACTGATTAGTACACTAGTGG | |
| pAG415GAL-ccdB promoter remover 2 | CCACTAGTGTACTAATCAGTATGGAGCTCCC |
a
F, forward; R, reverse.