Table 3.
In vitro development of NT reconstructs
| Cell line | No. of independent NT expts | No. of NT reconstructsa | No. (% ± SEM) |
||
|---|---|---|---|---|---|
| Morulae | Blastocysts | Total developmentb | |||
| F-jmjd2b, noninduced | 12 | 240 | 31 (13 ± 4) | 58 (24 ± 6) | 89 (37 ± 7) |
| F-jmjd2b, induced | 12 | 223 | 34 (15 ± 4) | 74 (33 ± 5)c | 108 (48 ± 5)d |
| M-jmjd2b, noninduced | 3 | 76 | 11 (14 ± 4) | 36 (47 ± 11) | 47 (62 ± 15) |
| M-jmjd2b, induced | 3 | 76 | 7 (9 ± 0.4) | 37 (49 ± 17) | 44 (58 ± 17) |
a
All data relate to reconstructs that extruded a single polar body and that were cultured in vitro until day 4.
b
Morulae and blastocysts.
c
P = 0.041 compared with noninduced F-jmjd2b cells determined by the Fisher 2-by-2 test.
d
P = 0.018 compared with noninduced F-jmjd2b cells determined by the Fisher 2-by-2 test.