Fig 7.

Overexpression of dn-HIF1α locally in adipose tissue increases peripheral use of lipid, decreases HFD-induced hepatic steatosis, and regulates circulating leptin levels. (A) Liver triglyceride (left) and cholesterol (right) in dn-HIF1α transgenic mice and their littermate controls after treatment with an HFD plus DOX for 8 weeks (n = 5 for each group). *, P < 0.05. (B) H&E staining for liver tissues in dn-HIF1α transgenic mice and their littermate controls. The bars represent 50 μm. (C) qPCR analysis of leptin in EWAT (left) and circulating leptin levels measured by ELISA (right) in dn-HIF1α transgenic mice and their littermate control mice after treatment with an HFD plus DOX for 8 weeks (n = 5 for each group). *, P < 0.05.