Fig 2.

Atypical PKCι association with TGF-β receptors. HEK293T cells expressing the indicated combinations of GFP-tagged PKCι, HA-tagged TGF-β type II receptors (TβRII), Flag-tagged TGF-β type I receptors (TβRI), wild-type Par6 (Par6 WT), or a mutant of Par6 that does not associate with TGF-β receptor (Par6-ΔPB1) were lysed immunoprecipitated (IP) with anti-HA antibodies. The immunoprecipitates were then subjected to SDS-PAGE and immunoblotted with anti-GFP (α-GFP), anti-HA, or anti-Flag antibodies as primary antibodies to visualize proteins that coprecipitated with TβRII (top panel). The nonspecific immunoglobulin heavy chain is indicated (IgG), and cell lysates are shown in the bottom panel.