Fig 6.

Knockdown of ZFP-1 by RNAi leads to germ line defects. (A) One gonad arm is shown for wild-type (top) and zfp-1(RNAi) (bottom) animals. Magnification, ×200. Chromatin is visualized by H2B::GFP, arrows point to oocyte nuclei, and an oogenesis defect is evident in zfp-1(RNAi) animals. DIC, differential interference contrast. (B) Multinucleated oocytes similar to those shown in panel A were observed during zfp-1 RNAi in an RNAi-sensitized [eri-1(mg366)] background. (C) Proximal germ line tumors in place of developing oocytes (arrow) were observed in some zfp-1(RNAi) animals. The example shown is from the RNAi experiment in the eri-1(mg366) background. Magnification, ×400. Arrowheads point to the vulva.