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Changes in the percentage of regulatory T cells in autoimmune patients before (Pre-IVIg) and following IVIg (Post-IVIg) therapy. Peripheral blood mononuclear cells were isolated from heparinized blood samples and CD4+CD25high Tregs were analyzed by flow cytometry (LSR II, BD Biosciences) by using fluorescence-conjugated monoclonal antibodies (BD Biosciences) The patients are represented by open circles. *, P<0.05 by Student-t-test.
