A, Bar graphs showing induction of miR-132 (Ai) and inhibition of its target p120RasGAP (Aii) in SVPs exposed to H/S. AntimiR-132 transfection (50nmol/L) suppressed miR-132 expression while inducing p120RasGAP expression (Aii). Control SVPs were transfected with scrambled sequence (Scr, 50nmol/L), or exposed to transfectionvehicle (V). B. Levels of miR-132 in SVP-CM. Data are means±SE from experiments performed in quadruplicates. *P<0.05, **P<0.01, and ***P<0.001 vs corresponding group under normoxia; §§P<0.01 and §§§P<0.001 vs vehicle; ##P<0.01 and ###P<0.001 vs Scr. C, Bar graphs showing miR-132 levels (Ci) and p120RasGAP protein expression (Cii) in HUVECs exposed to SVP-CM collected under different conditions described above. D, Representative images and bar graphs showing the ability of SVP-CM to enhance HUVEC network formation and abrogation of this effect by CM of antimiR-132-transfected SVPs. HUVECs were cultured on matrigel with SVP-CM for 24 hours. Data are means±SE of experiments performed in quadruplicates. ɸP<0.05; ɸɸP<0.01 vs unconditioned medium (UCM); ɸɸɸP<0.001; §§P<0.01 vs corresponding group under normoxic SVP-CM; ##P<0.01 and ###P<0.001 vs Scr.