Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| E. coli JM109 | recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ(lac-proAB)/F′[traD26 rpoAB+ laclq lacZΔM15] | Promega |
| B. contaminans | ||
| MS14 | Wild-type strain | 9 |
| MS14KC1 | ocfC::nptII derivative of MS14; Kmr | This study |
| MS14MT18 | ocfE::Tn5 derivative of MS14; Kmr | 6 |
| MS14KC1-R | Revertant strain of the ocfC::nptII mutant | This study |
| Plasmids | ||
| pBR325 | Cloning vector; Cmr Tcr Apr | 10 |
| pMLS7 | Expression vector of Burkholderia; Tpr | 11 |
| pGEM-T Easy | Cloning vector; Apr | Promega |
| pBSL15 | Kanamycin resistance gene cassette; Kmr | 12 |
| pKC1 | pGEM-T Easy carrying 1.5-kb PCR product containing the intact ocfC gene; Apr | This study |
| pKC2 | pGEM-T Easy containing 2.8-kb ocfC and nptII; Kmr | This study |
| pKC3 | pBR325 carrying 2.8-kb EcoRI fragment containing the intact ocfC gene; Kmr Tcr Apr | This study |
| pKC4 | pMLS7 carrying 1.5-kb PCR product containing the intact ocfC gene; Cmr Tpr | This study |
| pDP12 | pBR325 carrying 1.5-kb EcoRI fragment of pKC1 containing the intact ocfC gene; Kmr Tcr Apr | This study |
a
Kmr, kanamycin resistance; Apr, ampicillin resistance; Tpr, trimethoprim resistance; Cmr, chloramphenicol resistance.