Table 2.
Chemical shift values for the ocfC mutant product
| Amino acid | Chemical shift value(s)a |
|||
|---|---|---|---|---|
| HN | Hα | Hβ | Other proton(s) | |
| Asn1 | 8.13 | 4.53 [52.91] | 2.58, 2.36 [40.09] | γ-NH2, 7.23, 6.84 |
| BHN1 | 7.95 | 4.62 [58.79] | 4.03 [74.94] | γ-NH2, 7.20, 6.77; β-OH, 5.71 |
| NAA2 | 7.49 | C-2:CH2, 2.38 [39.98]; C-3:CH, 4.14 [47.49]; C-4:CH2, 1.77 [41.32] | ||
| 7.35 | C-2:CH2, 2.32 [43.78]; C-3:CH, 4.19 [47.49]; C-4:CH2, 1.39 [41.32] | |||
| Ser3 | 8.13 | 4.21 [58.90] | β-OH, 5.08 | |
| 8.18 | 4.18 [58.81] | β-OH, 5.08 | ||
| BHY4 | 8.02 | 4.18 [63.07] | 5.08 [73.89] | β-OH, 5.70 |
| DABA5 | 7.71 | 4.39 [53.86] | 2.11, 1.91 [32.78] | γ-H, 2.92 [32.78]; NH2, 7.74 |
| Gly6 | 7.93 | 3.81, 3.64 [45.11] | ||
| 7.85 | 3.87, 3.65 [45.11] | |||
| Asn7 | 8.36 | 4.58 [58.71] | 2.63, 2.41 [40.09] | γ-NH2, 7.38, 6.92 |
| 8.30 | 4.56 [52.91] | 2.63, 2.40 [40.09] | γ-NH2, 7.38, 6.92 | |
| Ser8 | 7.81 | 4.20 [58.70] | 3.62 [63.07] | β-OH, 5.69 |
a
Proton chemical shift values are from TOCSY and NOESY experiments. Chemical shifts in brackets are 13C values from the HSQC experiment.