Fig 1.

Effects of TA on the growth of Pseudomonas protegens Pf-5. Growth of Pf-5, assessed by measuring the optical density at 595 nm (OD₅₉₅), in MH medium containing various concentrations of TA at 25°C for 2 days. Arrows depict TA concentrations used in the transcriptomic studies. Error bars denote standard deviations. (Inset) Effects of TA on growth of Pf-5 assessed as CFU per ml after plating on Luria-Bertani agar plates containing streptomycin (100 μg/ml). Statistical analysis was performed using a t test (P ≤ 0.05). No statistically significant difference in growth of Pf-5 was observed between the nonamended and low-TA-amended (20 μg/ml) cultures. Concentrations of TA did not significantly alter the pH of the spent culture medium (control, pH 7.06; low-TA treatment, pH 7.01; and high-TA treatment, pH 6.99).