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SDS-PAGE analysis of partially purified PelF. Soluble fractions of PelF-producing and PelF-deficient strains were subjected to a HSMP column, and elution fractions were analyzed by SDS-PAGE. An arrow (thick) indicates the presence of His10-PelF. Three proteins coeluting with PelF were identified: A, ATP-dependent protease (GI 15595976); B, PA4657 (GI 15599852) of P. aeruginosa; C, Gcn5-related N-acetyltransferase (GI 239721) (best match to Serratia marcescens).
