Table 2.

Sequences of synthetic DNA fragments used in this study

DNA fragment	Sequencea
D301A	CCCGGGTGATCCCCAACGGCATCGCCCTCGATGCCTGGACCGGCGCCCTCGAACGGCGGCCGCCGGGGATTCCGCCGGTGGTCGGGCTGGTCGGCCGGGTAGTGCCGATCAAGGACGTGAAGACCTTCATCCGCGCCATGCGCGGGGTGGTCAGCGCGATGCCGGAGGCGGAGGGCTGGATCGTCGGTCCGGAGGAGGAAGATCCGGACTATGCCAGCGAATGCCGCAGCCTGGTGGCCAGCCTCGGCCTGCAG
D360A	CCCGGGTGATCCCCAACGGCATCGACCTCGATGCCTGGACCGGCGCCCTCGAACGGCGGCCGCCGGGGATTCCGCCGGTGGTCGGGCTGGTCGGCCGGGTAGTGCCGATCAAGGACGTGAAGACCTTCATCCGCGCCATGCGCGGGGTGGTCAGCGCGATGCCGGAGGCGGAGGGCTGGATCGTCGGTCCGGAGGAGGAAGCTCCGGACTATGCCAGCGAATGCCGCAGCCTGGTGGCCAGCCTCGGCCTGCAG
R325A	CCCGGGTGATCCCCAACGGCATCGATCTCGACGCCTGGACCGGCGCCCTCGAACGGCGGCCGCCGGGGATTCCGCCGGTGGTCGGGCTGGTCGGCGCCGTAGTGCCGATCAAGGATGTGAAGACCTTCATCCGCGCCATGCGCGGTGTGGTCAGCGCGATGCCGGAGGCGGAAGGCTGGATCGTCGGTCCGGAGGAGGAAGACCCGGACTATGCCAGCGAATGCCGCAGCCTGGTGGCCAGCCTCGGCCTGCAG
K330A	CCCGGGTGATCCCCAACGGCATCGATCTCGACGCCTGGACCGGCGCCCTCGAACGGCGGCCGCCGGGGATTCCGCCGGTGGTCGGGCTGGTCGGCCGGGTAGTGCCGATCGCCGATGTGAAGACCTTCATCCGCGCCATGCGCGGTGTGGTCAGCGCGATGCCGGAGGCGGAAGGCTGGATCGTCGGTCCGGAGGAGGAAGACCCGGACTATGCCAGCGAATGCCGCAGCCTGGTGGCCAGCCTCGGCCTGCAG
K333A	CCCGGGTGATCCCCAACGGCATCGATCTCGACGCCTGGACCGGCGCCCTCGAACGGCGGCCGCCGGGGATTCCGCCGGTGGTCGGGCTGGTCGGCCGGGTAGTGCCGATCAAGGATGTGGCCACCTTCATCCGCGCCATGCGCGGTGTGGTCAGCGCGATGCCGGAGGCGGAAGGCTGGATCGTCGGTCCGGAGGAGGAAGACCCGGACTATGCCAGCGAATGCCGCAGCCTGGTGGCCAGCCTCGGCCTGCAG

^aUnderlined and italicized nucleotides indicate PstI and SmaI restriction sites; underlined nucleotides indicate changes to introduce site-directed mutations; italicized and boldface nucleotides indicate silent mutations introduced to adjust the CG content, which was problematic during DNA synthesis; boldface nucleotides indicate silent mutations introduced to add a new restriction site, i.e., ClaI.