Fig 4.

(A) Production of Neu5Ac from 250 mM GlcNAc and 100 mM pyruvate over 44 h at 50°C using 10 mg (wet weight) of NanA- and Slr1975-displaying beads. NanA-PhaC plus PhaC-l-Slr1975 beads (5 mg [wet weight] each) yielded 14% ManNAC and 4.7% Neu5Ac conversion of the starting GlcNAc concentration. NanA-PhaC-l-Slr1975 beads (10 mg wet weight) yielded 2% ManNAc and 1.7% Neu5Ac conversion of the starting GlcNAc concentration. (B) Production of Neu5Ac from 100 mM GlcNAc and 100 mM pyruvate over 50 h at 30°C using 10 mg (wet weight) of NanA- and Slr1975-displaying beads. NanA-PhaC plus PhaC-l-Slr1975 beads (2.5 mg and 7.5 [wet weight], respectively) yielded 19% ManNAC and 22% Neu5Ac conversion of the starting GlcNAc concentration. Error bars represent ± 1 standard deviation; n = 3.