Fig 5.

In vitro analysis of Ef-OAD stability as a function of pH. E. faecalis cell extracts were first incubated in batch with a neutravidin resin. After the binding step, the resin was washed (W; lane 3) with 1 ml 150 mM citrate, pH 7.0, and eluted (lanes 4 to 8) with 60-μl aliquots of citrate (A) or phosphate buffers (B) according to the indicated pH range. As a final step, samples resuspended in Laemmli loading buffer were boiled (100°C). FT, flowthrough. M, molecular markers. An asterisk corresponds to avidin released from the column.