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. 2013 May;79(9):2882–2890. doi: 10.1128/AEM.03980-12

Fig 6.

Fig 6

Ef-OAD complex expression in E. coli. (A) SDS-PAGE of first and second elution fractions (E1 and E2) of an avidin affinity chromatography purification of E. coli cell extracts from strains carrying different Ef-OAD subunits (pET-ADB and pET-AHDB). Relevant bands are indicated by arrowheads. W, third washing step. (B) Immunoblot detection of Ef-A expression from crude extracts of induced cells harboring pET-ADB and pET-AHDB plasmids.