Fig 1.
Plasmids used for BiFC analysis. A system based on two broad-host-range plasmids (pBBR1MCS-2 [22] and pCM62 [23]) was constructed to carry out BiFC in an R. eutropha background. N-terminal (YN) and C-terminal (YC) parts of eYfp together with a C-terminal MCS to clone the protein of interest were inserted in the two plasmids, respectively. The resulting plasmids, pBBR-YN-C1 and pCM-YC-C1, can be transferred to R. eutropha or other Gram-negative bacteria via conjugation and used to express BiFC fusions from the l-arabinose-inducible PBAD promoter.