Fig 6.

Determination of quaternary structures of PhaP5 and PhaM. Analytical gel filtration was carried out on a Superdex-200 column (for details, see Materials and Methods). Calibration of the column (upper left) was performed using the following standard proteins: thyroglobulin (670 kDa), gamma globulin (158 kDa), conalbumin (75 kDa), ovalbumin (44 kDa), carbonic anhydrase (29 kDa), myoglobin (17 kDa), RNase (13.7 kDa), aprotinin (6.5 kDa), and vitamin B₁₂ (1.35 kDa). Elution profiles of selected standard proteins (dashed line), purified PhaP5-His₆ (gray line, V_e = 15.98 ml), and purified PhaM-His₆ (black line, V_e = 11.21 ml) are shown. The void volume of the column (blue dextran) was determined as 8.41 ml.