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SDS-PAGE results for the soluble fractions of cell extracts of E. coli BL21(DE3)(pLysS) carrying either pET_p4h1or (lanes 1 and 2) or pET_p4h1of (lanes 3 and 4), grown at 25°C or 30°C in M9 medium with 0.5% (wt/vol) glucose in the absence (lanes 1 and 3) or presence (lanes 2 and 4) of 20 mM proline. Lane M, protein size marker.
