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Comparison of specific activities of resting whole cells (a), permeabilized cells (b), and cell extracts (c) of E. coli BL21(DE3)(pLysS) carrying either pET_p4h1or or pET_p4h1of, grown in M9 medium at 25°C with 0.5% (wt/vol) glucose in the presence or absence of 20 mM proline. See Materials and Methods for experimental details.
