Fig 4.

Genetic organizations of 3′-side boundary regions of the cps loci. (A) The 3′-side boundary regions of cps2, cps8, cps9, and cps10 loci. The serotype 8 reference strain 14363 possesses genes corresponding to SSU0563 (glf) to SSU0565 of S. suis serotype 2 strain P1/7 (cps8N-P) and the putative remnants of a glf homologue (cps8F-G) in the cps gene cluster. The serotype 10 reference strain 4417 and serotype 9 strain D12 also harbor glf and SSU0565 homologues in the cps gene clusters. On the other hand, serotype 9 reference strain 22083 does not have these homologues in the cps gene cluster. (B) The 3′-side boundary regions of cps6, cps16, and cps21 loci. The glf homologues are present in the cps gene clusters of serotype 6 and 16 reference strains, but the genes corresponding to SSU0564 and SSU0565 are absent from the gene clusters. Homologues of a putative transposase gene located downstream of glf in the serotype 21 reference strain are present upstream of aroA in serotype 6 and 16 reference strains. Conserved regions by BLASTN analysis are represented by light blue blocks. The locus tag numbers or gene names (“cps” omitted) are appended to the corresponding genes.