Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Agromonas oligotrophica strains | ||
| S58T | Wild-type strain derived from paddy soil | 1 |
| S58::dsRed | This study | |
| S58::gusA | This study | |
| S42 | Wild-type strain derived from paddy soil | 1 |
| S55 | Wild-type strain derived from paddy soil | 1 |
| S72 | Wild-type strain derived from paddy soil | 1 |
| S80 | Wild-type strain derived from paddy soil | 1 |
| Bradyrhizobium sp. strains | ||
| ORS278 | Wild-type strain derived from stem nodule of A. indica | 7 |
| BTAi1 | Wild-type strain derived from stem nodule of A. sensitiva | 7 |
| G14130 | Slow-growing oligotrophic bacterium derived from grassland soil | 32 |
| HWK12 | 2,4-d-degrading bacterium derived from Hawaiian soil | 44 |
| HW13 | 2,4-d-degrading bacterium derived from Hawaiian soil | 44 |
| Bradyrhizobium japonicum strains | ||
| USDA110 | Soybean bradyrhizobium | 31 |
| USDA122 | Soybean bradyrhizobium | 6, 45 |
| USDA6 | Soybean bradyrhizobium; type strain of B. japonicum | 11 |
| NC4 | Soybean bradyrhizobium | 6 |
| NC6 | Soybean bradyrhizobium | 6 |
| NK2 | Soybean bradyrhizobium | 6 |
| T7 | Soybean bradyrhizobium | 6 |
| T9 | Soybean bradyrhizobium | 6 |
| Bradyrhizobium elkanii strain | ||
| USDA76 | Soybean bradyrhizobium; type strain of B. elkanii | 45 |
| Rhodopseudomonas palustris strain | ||
| CGA009 | Photosynthetic bacterium | 46 |
| Escherichia coli strain | ||
| DH5α | recA; cloning strain | Toyobo Inc. |
| Plasmids | ||
| pmTn5SSgusA20 | Plasmid used for transposon insertion; gusA, Apr, Smr, Spr | 47 |
| pBjGroEL4::dsRed2 | Plasmid used for transposon insertion; dsRed, Apr, Smr, Spr | See Fig. S1 in the supplemental material |
| pRK2013 | ColE replicon carrying RK2 transfer genes; Kmr | 48 |