Expression of the cryD gene. (A) Effect of light on cryD and wcoA mRNA levels in wild-type F. fujikuroi. The data show qRT-PCR analyses of total RNA samples from the wild type grown in the dark or exposed for 15 min, 30 min, 1 h, 2 h, or 4 h to 7 W m−2 white light. Relative levels are referred to the values for each gene in the dark. All data show means and standard deviations for three measurements from three independent experiments. (B) Relative levels of mRNAs for genes carRA and carB in four carotenoid-overproducing mutants (carS) grown in the dark, numbered 1 to 4 (1, SF114; 2, SF115; 3, SF116; and 4, SF134). The mRNA levels were referred to those of the wild type under the same conditions (C).