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Example of one of the specificity tests for Ophiostoma brunneo-ciliatum real-time LAMP assay. In the amplification plot (A), besides the positive controls for O. brunneo-ciliatum (isolates CBS 117591 and CBS 117571), O. clavatum isolate CMW 37984 and Hyalorhinocladiella macrospora isolate CBS 267.53 produced positive responses. However, in the annealing plot (B), nonspecific amplifications showed an annealing temperature that differed by more than 1°C from the specific ones.
