Table 3.
LAMP assay target | Primer | Sequence (5′–3′) | Target region length (bp) |
---|---|---|---|
Ophiostoma clavatum | F3 | CCTCGTTGAAGTAGACGCTC | 176 |
B3 | GATTCCGATCTACGGCTCC | ||
FIP | GTTGGACGTTGGACGCCGCGAGGCGCTCCAGCTGGAGA | ||
BIP | GAAGTGGGAGAATACATACACGCCATGGCTGAACTCAACACTGAC | ||
F-Loop | ACCGCAGCTAACGTAATGTCC | ||
B-Loop | GCTGTCAAGGCCGTGCTC | ||
O. brunneo-ciliatum | F3 | GACCGAAAGGACCGGCAC | 217 |
B3 | GCTCCACCGCGGCATGA | ||
FIP | CTGTCCCTAGGTACAACGGCACAGATCGACAAGGACGGCAC | ||
BIP | CCACGACCAACATCAGGGGAGACAGGCAGCAGATTTCCGGC | ||
F-Loop | CCTCTGGCAACAAGTACGTG | ||
B-Loop | GCTGTCAAGGCCGTGCTC | ||
Ips acuminatus | F3 | CATTTCATGGAGCTCAAATTTC | 182 |
B3 | TCCTGTAAAAAGTGGAAATCATT | ||
FIP | TCCGGTTAAACCTCCTAGAGTAAATAATCCCTCAAGACTTTGATCT | ||
BIP | ATGTAGTTGCCCATTTCCATTATGTGGACAATTCCTGCAATAATAG | ||
B-Loop | ACTTTCAATAGGAGCTGTATTTG |