Table 1.

Mean frequency of primer homology to UNITE database ITS sequences

rDNA	Primer	Sequence (5′→3′)	Referencea	Non-3′ mismatch	3′ mismatch	3′ gaps	Non-3′ gaps	Weighted scoreb
SSU	BITS	ACCTGCGGARGGATCA		2.511	0.886	0.028	0.008	2.642
	ITS1	TCCGTAGGTGAACCTGCGG	24	3.940	1.153	0.055	0.003	3.431
	ITS5	GGAAGTAAAAGTCGTAACAAGG	24	6.458	1.038	0.053	0.000	4.173
	ITS1-F_KYO2	TAGAGGAAGTAAAAGTCGTAA	16	6.111	1.448	0.027	0.002	4.238
	ITS1-F_KYO1	CTHGGTCATTTAGAGGAASTAA	16	6.353	1.508	0.138	0.003	5.340
	NSI1	GATTGAATGGCTTAGTGAGG	23	6.108	1.498	0.056	0.001	5.565
	ITS1F	CTTGGTCATTTAGAGGAAGTAA	22	6.858	1.598	0.108	0.005	5.910
	NSA3	AAACTCTGTCGTGCTGGGGATA	23	7.225	1.519	0.111	0.005	6.077
5.8S Reverse	ITS2_KYO1	CTRYGTTCTTCATCGDT	16	0.429	0.132	0.013	0.000	0.425
	ITS2_KYO2	TTYRCTRCGTTCTTCATC	16	0.438	0.146	0.010	0.001	0.492
	B58S3	GAGATCCRTTGYTRAAAGTT		0.533	0.165	0.012	0.000	0.534
	58A2	CTGCGTTCTTCATCGAT	23	0.495	0.187	0.011	0.001	0.545
	ITS2	GCTGCGTTCTTCATCGATGC	24	0.644	0.195	0.027	0.001	0.704
5.8S Forward	ITS3_KYO2	GATGAAGAACGYAGYRAA	16	0.502	0.109	0.011	0.000	0.399
	gITS7	GTGAATCATCGARTCTTTG	15	0.506	0.135	0.014	0.000	0.461
	ITS3_KYO1	AHCGATGAAGAACRYAG	16	0.402	0.142	0.012	0.001	0.478
	fITS7	GAACACAGCGAAATGTGA	15	0.575	0.135	0.016	0.000	0.497
	58A2	ATCGATGAAGAACGCAG	23	0.502	0.185	0.012	0.001	0.531
	58A1	GCATCGATGAAGAACGC	23	0.514	0.191	0.010	0.001	0.569
	ITS3	GCATCGATGAAGAACGCAGC	24	0.667	0.198	0.027	0.001	0.640
	fITS9	GAACACAGCGAAATGTGA	15	1.629	1.085	0.016	0.001	1.901
LSU	ITS4_KYO1	TCCTCCGCTTWTTGWTWTGC	16	4.274	1.107	0.039	0.002	3.846
	ITS4	TCCTCCGCTTATTGATATGC	24	4.489	1.480	0.057	0.004	4.070
	ITS4_KYO2	RBTTTCTTTTCCTCCGCT	16	4.181	1.351	0.015	0.001	4.253
	ITS4_KYO3	CTBTTVCCKCTTCACTCG	16	3.804	1.446	0.016	0.002	4.703
	NLB4	GGATTCTCACCCTCTATGAC	23	6.410	0.819	0.025	0.002	5.228
	ITS4B	CAGGAGACTTGTACACGGTCCAG	22	7.528	1.694	0.127	0.005	6.198

^aIn cases where no reference is listed, this study is meant.

^bWeighted score = (non-3′ mismatches × 0.4) + 3′ mismatches + non-3′ gaps + (3′ gaps × 3.0) + (final 3′ base mismatch × 3.0).