Table 1.

Composition of glucose-limited mineral medium E for the cultivation of Listeria

Compound	Mass concn	Molar concn
EDTA	1.4612 g liter−1	5.00 mmol liter−1
KH2PO4	0.656 g liter−1	4.82 mmol liter−1
Na2HPO4·H2O	2.047 g liter−1	12.80 mmol liter−1
MgSO4·7H2O	1.55 g liter−1	6.29 mmol liter−1
(NH4)2SO4	7.07 g liter−1	53.50 mmol liter−1
Glucose	2.5 g liter−1	13.88 mmol liter−1
MOPS	20.93 g liter−1	100.01 mmol liter−1
Trace elementsa
CaCO3	80 mg liter−1	0.40 mmol liter−1
FeCl3·6H2O	38.7 mg liter−1	143.18 μmol liter−1
MnCl2·4H2O	5.75 mg liter−1	29.05 μmol liter−1
CuSO4·5H2O	0.73 mg liter−1	2.92 μmol liter−1
CoCl2·6H2O	0.65 mg liter−1	2.73 μmol liter−1
ZnO	2 mg liter−1	24.57 μmol liter−1
H3BO3	0.62 mg liter−1	10.03 μmol liter−1
EDTA·Na4·2H2O	396 mg liter−1	0.94 mmol liter−1
MgCl2·6H2O	67.1 mg liter−1	0.33 mmol liter−1
Na2MoO4·2H2O	5.2 mg liter−1	21.49 μmol liter−1
Vitaminsb
Biotin	20 μg liter−1	81.86 pmol liter−1
Folic acid	20 μg liter−1	45.31 pmol liter−1
Pyridoxine	100 μg liter−1	591.09 pmol liter−1
Thiamine	50 μg liter−1	166.22 pmol liter−1
Riboflavin	50 μg liter−1	132.85 pmol liter−1
Niacin	50 μg liter−1	406.14 pmol liter−1
Cobalamin	50 μg liter−1	36.89 pmol liter−1
Pantothenic acid	50 μg liter−1	228.07 pmol liter−1
4-Aminobenzoic acid	50 μg liter−1	364.60 pmol liter−1
Lipoic acid	50 μg liter−1	242.34 pmol liter−1
Nicotinamide	50 μg liter−1	409.42 pmol liter−1
Amino acidsa
Cysteine	100 mg liter−1	825.37 μmol liter−1
Glutamine	600 mg liter−1	4105.53 μmol liter−1
Methionine	100 mg liter−1	670.19 μmol liter−1
Histidine	100 mg liter−1	644.52 μmol liter−1
Tryptophan	100 mg liter−1	489.66 μmol liter−1
Leucine	100 mg liter−1	762.35 μmol liter−1
Isoleucine	100 mg liter−1	762.35 μmol liter−1
Valine	100 mg liter−1	853.63 μmol liter−1
Arginine·HCl	120.9 mg liter−1	573.91 μmol liter−1

^aAdded from 100× stock solution.

^bAdded from 1,000× stock solution.