Table 2.

Viable counts on MA and real-time PCR enumeration of P. phosphoreum in naturally contaminated MAP salmon steaks (log CFU/g) and their relative accuracy

Sample	Real-time PCR (log CFU/g)	CT valuesa	Viable count (log CFU/g)b	Relative accuracy (%)c
1	3.32	30.57 ± 0.56	2.88	115.3
2	5.37	24.29 ± 0.77	5.49	97.8
3	7.13	18.89 ± 0.14	7.03	101.4
4	7.34	18.27 ± 0.17	7.38	99.5
5	3.47	30.11 ± 0.10	3.57	97.2
6	3.85	28.93 ± 0.47	4.42	87.1
7	7.06	19.11 ± 0.30	7.06	100
8	7.66	17.28 ± 0.20	7.61	100.7
9	7.47	17.85 ± 0.21	7.31	102.2
10	3.84	28.96 ± 0.19	4.54	84.7
11	5.36	24.32 ± 0.16	5.60	95.6
12	7.40	18.06 ± 0.17	7.20	102.8
13	6.76	20.02 ± 0.32	7.29	92.8

^aMean and standard deviation of three C_T values.

^bViable counts were determined using MA and identification of colonies.

^cDegree of correspondence between the results obtained with a conventional plating technique and with a real-time PCR assay. The relative accuracy is expressed as the percentage of numbers of log (CFU/g) calculated by the real-time PCR assay versus the conventional method (45).