Table 3.

Identification of 50 isolates from MA plates using partial 16S rRNA gene sequencing and P. phosphoreum identification by real-time PCR method

Sample	Isolate	Species or genus identification by partial 16S rRNA gene sequencing	P. phosphoreum identification using real-time PCR methoda
1	1	Serratia fonticola	−
	2	Pseudomonas sp.	−
	3	Pseudomonas sp.	−
	4	Pseudomonas sp.	−
	5	Pseudomonas sp.	−
	6	Acinetobacter sp.	−
	7	Pseudomonas sp.	−
	8	Pseudomonas sp.	−
	9	Pseudomonas sp.	−
	10	Pseudomonas sp.	−
	11	Shewanella sp.	−
	12	Pseudomonas sp.	−
	13	Myroides odoratimimus	−
	14	Acinetobacter sp.	−
	15	Nonworkable sequence	−
	16	Shewanella sp.	−
	17	P. phosphoreum	+
	18	Nonworkable sequence	−
	19	Pseudomonas sp.	−
	20	Pseudomonas sp.	−
2	1	P. phosphoreum	+
	2	P. phosphoreum	+
	3	P. phosphoreum	+
	4	P. phosphoreum	+
	5	P. phosphoreum	+
	6	P. phosphoreum	+
	7	P. phosphoreum	+
	8	P. phosphoreum	+
	9	P. phosphoreum	+
	10	Shewanella sp.	−
3	1	P. phosphoreum	+
	2	P. phosphoreum	+
	3	Nonworkable sequence	+
	4	P. phosphoreum	+
	5	P. phosphoreum	+
	6	Shewanella sp.	−
	7	P. phosphoreum	+
	8	Shewanella sp.	−
	9	P. phosphoreum	+
	10	P. phosphoreum	+
4	1	Shewanella sp.	−
	2	Nonworkable sequence	+
	3	Nonworkable sequence	+
	4	P. phosphoreum	+
	5	P. phosphoreum	+
	6	P. phosphoreum	+
	7	P. phosphoreum	+
	8	P. phosphoreum	+
	9	P. phosphoreum	+
	10	Photobacterium sp.	+

^a+, amplification product with a T_m of 84°C obtained in the method's range of use; −, no PCR product obtained in the method's range of use.