Table 1.
Luminescence-up mutants
| Disrupted gene (ORF) | Independent mutant(s)a | Putative functionb |
|---|---|---|
| arcA (VF2120) | SLV41 | Regulator, redox-responsive TCRS |
| arcB (VF2122) | NL3, SLV41, SLV19, NL5, SLV36, NL6, SLV33 | Sensor, redox-responsive TCRS |
| acnB (VF2158) | NRc | Aconitase (TCA cycle enzyme) |
| topA (VF1051) | EMH12, EMH13 | Topoisomerase I (relieves negative supercoils) |
| lonA (VF2352) | SLV32, SLV39, EMH6 | ATP-dependent protease |
| pstA (VF1984) | SLV10 | High-affinity phosphate transport |
| pstC (VF1985) | SLV30 | High-affinity phosphate transport |
| hns (VF1631) | SLV15 | Nucleoid-associated DNA-binding protein, global repressor |
| tRNAMet (VFIRNA222) | EMH7 | Methionine tRNA |
| tRNAThr (VFIRNA003) | NL4 | Threonine tRNA |
| tfoY (VF1573) | NL1, EMH9 | Regulator of transformation competence |
| phoQ (VF1397) | SLV16, SLV43, EMH3 | Sensor, Mg2+-responsive TCRS |
| guaB (VF0637) | EMH5 | Inositol-5-monophosphate dehydrogenase (purine metabolism) |
| ainS (VF1037) | NR | Octanoyl homoserine lactone synthase |
a
Mutants previously identified as brighter than wild type in culture (12). Underlining indicates the representative strain for each locus reported here.
b
TCRS, two-component regulatory system; TCA, tricarboxylic acid.
c
NR, not reported, either due to a high rate of suppressor mutations (acnB mutants) or because symbiotic phenotypes were previously published (ainS mutants).