Fig 1.

Genomic organization of the ca. 35-kb island harboring arsenic and cadmium resistance cassettes in ECIa strain Scott A. The DNA sequence of Scott A genome contig 5 (accession no. AFGI01000005.1) was retrieved from the NCBI database, annotated with the xBASE bacterial genome annotation service (http://www.xbase.ac.uk/annotation/) using F2365 as the reference genome (29–34), and analyzed with DNA and protein BLAST (35) and NCBI's Conserved Domain Search (http://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi) (36). This annotation was later updated based on that available in NCBI (accession no. CM001159.1). The Scott A genomic region was compared with pLI100 and F2365 using the online Artemis Comparison Tool (WebACT; http://www.webact.org/WebACT/home) (37). Genes and pseudogenes are shown as arrows and stippled arrows, respectively. The conserved flanking genes in L. monocytogenes are shown in black, and other genes in the 35-kb insertion are in white. Flanking open reading frames (ORFs) on pLI100 are in a grid pattern. Homologous regions are represented in gray. Arrows above and below selected ORFs indicate the locations and orientations of primers (Table 2).