Table 2.
Results of qPCR on DNA extracted from bacterial isolates from commercial products, control strain ATCC 53103, and Lactobacillus rhamnosus yoba 2012a
| Cultivation condition/sample no. | Product 2B |
Product 4 |
ATCC 53103 |
Product 3/L. rhamnosus yoba 2012 |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
map |
spaC |
map |
spaC |
map |
spaC |
map |
spaC w |
|||||||||
| CT | Quantity | CT | Quantity | CT | Quantity | CT | Quantity | CT | Quantity | CT | Quantity | CT | Quantity | CT | Quantity | |
| RA/1 | 17.5 | 3.0E+06 | 33.8 | 3.46E+02 | 18.0 | 2.3E+06 | 35.2 | 1.5E+02 | 17.5 | 3.11E+06 | 18.3 | 3.7E+06 | 17.6 | 2.9E+06 | 18.6 | 3.2E+06 |
| RA/2 | 17.7 | 2.7E+06 | 34.0 | 3.00E+02 | 17.6 | 2.9E+06 | 18.5 | 3.4E+06 | 17.5 | 3.04E+06 | 18.4 | 3.6E+06 | 17.8 | 2.6E+06 | 18.7 | 2.9E+06 |
| RA/3 | 17.5 | 3.0E+06 | 18.4 | 3.46E+06 | 17.5 | 3.0E+06 | 35.9 | 9.6E+01 | 17.5 | 2.96E+06 | 18.3 | 3.8E+06 | 17.7 | 2.7E+06 | 18.7 | 2.9E+06 |
| RA/4 | 17.2 | 3.6E+06 | 32.5 | 7.53E+02 | 16.6 | 5.0E+06 | 17.5 | 6.0E+06 | ND | ND | ND | ND | ND | ND | ND | ND |
| RN/1 | 17.4 | 3.2E+06 | 18.2 | 3.92E+06 | 27.2* | 9.9E+03 | 29.8 | 3.8E+03 | 17.7 | 2.77E+06 | 18.5 | 3.4E+06 | 17.4 | 3.1E+06 | 18.3 | 3.7E+06 |
| RN/2 | 17.3 | 3.4E+06 | 19.4 | 1.91E+06 | 27.2* | 1.0E+04 | 30.5 | 2.4E+03 | 17.4 | 3.14E+06 | 18.3 | 3.9E+06 | 17.8 | 2.6E+06 | 18.6 | 3.1E+06 |
| RN/3 | 17.7 | 2.7E+06 | 32.2 | 8.78E+02 | 17.2 | 3.6E+06 | 35.2 | 1.5E+02 | 17.6 | 2.81E+06 | 18.1 | 4.2E+06 | 17.6 | 2.8E+06 | 19.0 | 2.4E+06 |
| RN/4 | 17.1 | 3.8E+06 | 34.0 | 3.02E+02 | 17.4 | 3.1E+06 | OR | ND | ND | ND | ND | ND | ND | ND | ND | |
| MA/1 | 17.4 | 3.3E+06 | 36.6 | 6.56E+01 | 17.7 | 2.6E+06 | 35.2 | 1.5E+02 | 17.6 | 2.94E+06 | 18.4 | 3.5E+06 | 17.5 | 3.0E+06 | 18.4 | 3.5E+06 |
| MA/2 | 17.6 | 2.8E+06 | 36.0 | 9.14E+01 | 17.7 | 2.8E+06 | OR | 17.5 | 3.00E+06 | 18.4 | 3.5E+06 | 17.7 | 2.8E+06 | 18.6 | 3.2E+06 | |
| MA/3 | 17.6 | 2.9E+06 | OR | 17.7 | 2.6E+06 | OR | 17.2 | 3.68E+06 | 18.1 | 4.2E+06 | 17.7 | 2.6E+06 | 18.6 | 3.1E+06 | ||
| MA/4 | 17.4 | 3.2E+06 | 37.5 | 3.65E+01 | 17.5 | 3.1E+06 | 34.8 | 1.9E+02 | ND | ND | ND | ND | ND | ND | ND | ND |
| MN/1 | 17.3 | 3.4E+06 | 36.6 | 6.38E+01 | ND | ND | ND | ND | 17.4 | 3.27E+06 | 18.5 | 3.3E+06 | 17.3 | 3.4E+06 | 18.5 | 3.3E+06 |
| MN/2 | 29.1* | 3.2E+03 | 32.0 | 1.03E+03 | ND | ND | ND | ND | 17.8 | 2.46E+06 | 18.9 | 2.6E+06 | 17.8 | 2.5E+06 | 18.9 | 2.6E+06 |
| MN/3 | 17.7 | 2.6E+06 | 38.6 | 1.90E+01 | ND | ND | ND | ND | 17.8 | 2.55E+06 | 18.9 | 2.6E+06 | 17.9 | 2.3E+06 | 19.0 | 2.4E+06 |
| MN/4 | OR* | OR | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ||
a
Abbreviations used cultivation conditions in column 1:R, Rogosa agar; M, MRS agar; A, aerobic; N, anaerobic. Other abbreviations: OR, out of range; ND, not determined. Quantities are expressed in fg/μl. *, the CT of the control gene map is much larger than the average value of 17.5; hence, no mathematical comparison of the data is possible (see the text).