Expression of cytochrome P450. Lane 1, uninduced clone; lane 2, induced clone expressing Bpro_5301 (47.49 kDa), Bpro_5300 (43.18 kDa), and Bpro_5299 (11.29 kDa); lane 3, clone expressing Bpro_5301 with a 6× His tag (48.32 kDa); lane 4, clone expressing Bpro_5299 with a 6× His tag (12.12 kDa); lane 5, clone expressing LacZ (120 kDa).