Table 1.
Oxygen uptake by resting cells of JS666a
| Test substrate (concn [mM]) | Oxygen uptake (nmol min−1 mg−1 protein) after growth on: |
||||
|---|---|---|---|---|---|
| cDCE | DCA | Cyclohexanone | Glycolate | Succinate | |
| cDCE (1.0) | 13 ± 0 | 6 ± 2 | <1 | <1 | <1 |
| 1,2-DCA (1.0) | 11 ± 0 | 23 ± 6 | <1 | <1 | <1 |
| 2-Chloroethanol (1.0) | 7 ± 2 | 13 ± 0 | <1 | <1 | <1 |
| Chloroacetaldehyde hydrate (0.5) | 55 ± 1 | 47 ± 0 | 81 ± 6 | 39 ± 2 | 27 ± 5 |
| Dichloroacetaldehyde hydrate (0.5) | 18 ± 2 | 12 ± 0 | 6 ± 4 | 7 ± 1 | 3 ± 0 |
| Cyclohexanone (0.5) | 10 ± 1 | 1 ± 2 | 276 ± 36 | <1 | <1 |
| Chloroacetate (1.0) | 2 ± 0 | 26 ± 8 | <1 | 7 ± 2 | 2 ± 1 |
| Dichloroacetate (1.0) | ND | 10 ± 3 | |||
| Glycolate (1.0) | 7 ± 2 | 14 ± 2 | <1 | 95 ± 1 | 6 ± 2 |
| Succinate (1.0) | 7 ± 1 | 32 ± 1 | 41 ± 1 | ||
a
Oxygen uptake by resting cells of JS666 grown with various substrates. Reaction mixtures contained substrate, cells (0.29 to 0.31 mg of protein), and air-saturated phosphate buffer (20 mM, pH 7.2) to a final volume of 1.85 ml. cDCE, 1,2-DCA, and cyclohexanone were added from a 1.0 M stock in acetone, and the other substrates were dissolved in water. Acetone did not affect the rates of oxygen uptake. Data represent means and standard deviations from at least two measurements. ND, not determined; blank cell, not tested.