Fig 6.
Comparison of IMED device and direct colony counting sensitivity for detection of Salmonella serovars derived from whole blood of septic mice. BALB/c mice were infected i.p. (103 CFU) with S. Typhimurium ATCC 14028 (circles) or S. Choleraesuis χ3246 (boxes). (A) Blood collected from the tail vein throughout the 5-day infection time course was assayed for bacteria via the IMED device or by direct colony counting (detection limit, ∼100 CFU/ml). + and −, IMED signal detection and no detection, respectively. (B) Bacterial colonization of the liver and spleen (numbers of CFU) at days 1, 3, and 5 day postinfection was determined by direct colony count (detection limits, <40 CFU for spleen and <20 CFU for liver).