Table 1.
LAMP primers that amplify the Salmonella recF target region for strain discriminationa
| Primer | Sequence 5′–3′ | Gene position |
|---|---|---|
| FIP (F2 + F1c) | GATAAGCAGGGCGACAGCAA-CAGCTCCGCGATCTTGTG | 794–813 + 751–768 |
| BIP (B2 + B1c) | AGCCAATCGACGTCTCACGC-AGCAGGAAGCGTTTGTTCTT | 822–841 + 865–884 |
| B3 | CCTCCGGCGTAATCAACTG | 714–732 |
| F3 | GCCGCGTGATTCGTCATG | 885–902 |
a
The LAMP primer set used in these studies consists of two inner (FIP and BIP) and two outer (F3 and B3) primers that amplify the target region of the recF gene in the vast majority of Salmonella serovars. The products differ significantly in their single-stranded loop regions, allowing strain-specific discrimination (Fig. 2A and C). FIP comprises regions F2 and F1c (F2 + F1c) within the recF gene (Fig. 2A); F1c indicates the sequence complementary to the F1 region. Similar notation is used for the BIP primer.