Table 2.
Sequences for Salmonella serovar-specific E-DNA target regions, synthetic LAMP target amplicons, and E-DNA probes
| DNA and serovar | Sequencea (5′–3′) | Gene position |
|---|---|---|
| E-DNA target region | ||
| S. Typhimurium | CTTCGCTCTGTAATCGCCCGTG | 843–864 |
| S. Choleraesuis | CTTCGCCCTGCAGGCGCCCGTG | 843–864 |
| Synthetic LAMP target amplicons | ||
| S. Typhimurium barbell | GATAAGCAGGGCGACAGCAAGCTCCGCGATCTTGTGACCGTCGGTACCGTCGATACGGACCTTGCTGTCGCCCTGCTTATCTTTGGTTAAGCCAATCGACGTCTCACGCTCTTCGCTCTGTAATCGCCCGTGAAGAACAAACGCTTCCTGCT GCGTGAGACGTCGATTGGCT | |
| S. Choleraesuis barbell | GATAAGCAGGGCGACAGCAAGCTCCGCGATCTTGTGACCGTCGGTACCGTCGATACGGACCTTGCTGTCGCCCTGCTTATCTTTGGTTAAGCCAATCGACGTCTCACGCTCTTCGCCCTGCAGGCGCCCGTGAAGAACAAACGCTTCCTGCTGCGTGAGACGTCGATTGGCT | |
| Nonspecific barbell | AGCCAATCGACGTCTCACGCAGCAGGAAGCGTTTGTTCTTCACGGGCGATTACAGAGCGAAGAGCGTGAGACGTCGATTGGCTTAACCAAAGATAAGCAGGGCGACAGCAAGGTCCGTATCGACGGTACCGACGGTCACAAGATCGCGGAGTTGCTGTCGCCCTGCTTATC | |
| E-DNA probe | ||
| S. Typhimurium | HS-trihexylthiol-CACGGGCGATTACAGAGCGAAG-(CH2)7-NH-MB | |
| S. Choleraesuis | HS-trihexylthiol-CACGGGCGCCTGCAGGGCGAAG-(CH2)7-NH-MB | |
a
Strain differences are in bold, and binding regions are underlined. MB, methylene blue.