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RT-qPCR results for the target gene ethC from RNA samples of strain L108 cells exponentially growing on fuel oxygenate ethers as indicated. Normalized expression (ΔΔ quantification cycle [Cq]) with standard deviations determined from results obtained with cDNA of MTBE cultures as the control and expression of 16S rRNA and mpdJ genes as the references. Also shown are MTBE degradation rates in nmol min−1 mg biomass−1 in resting cells derived from the respective ether cultures.
