Fig 3.

MTBE-to-glucose shift experiments. (A) MTBE degradation and TBA accumulation rates in nmol min⁻¹ mg biomass⁻¹ of strain L108 cells pregrown on MTBE (inoculum) and after two to five generations (G2 to G5) of growth on glucose, as indicated. (B) RT-qPCR (cDNA) and qPCR (DNA) results for the target genes ethC, mdpJ, and hcmA from DNA and RNA samples of strain L108 cells pregrown on MTBE (inoculum) and after two and five generations (G2, G5) on glucose. Normalized expression levels (ΔΔC_q) were calculated by normalization with control samples (DNA and cDNA from inoculum samples, respectively) and the 16S rRNA gene as reference.