Fig 4.
Prediction of promoter regions upstream of the ethA gene. A sigma factor binding site that possessed the −10 and −35 regions characteristic for σ70-initiated transcription (score −7.4, E value 2.2) was detected only in strain L108. For inspection, the 468- and 186-bp intergenic spacers upstream of ethA in strains L108 and IFP 2001, respectively, were analyzed using the prokaryotic promoter prediction tool at http://bioinformatics.biol.rug.nl/websoftware/ppp/. Highlighted in bold are the −35 and −10 regions, the +1 transcription start, the ribosomal binding site (RBS), and the translation start of ethA. Boxes indicate identical nucleotide sequences in the two strains.