Table 1.
Primer pairs used for standard PCRs and qPCRs
| Primer name | Sequence (5′ → 3′) | Target gene | Length (bp) |
|---|---|---|---|
| iso-for | TCGGTCCGGCATGGGACCTGAT | L108 isomerase upstream of ethA | 1,164 |
| iso-rev | AGGCGCGGAAGGACCCTG | ||
| ethAup-for | TCGGTCCGGCATGGGACCTGAT | eth promoter region | 1,618 |
| ethA-rev1 | ATCGCGCAGCGACATCGCTGCCTGCA | ||
| ethA-for | CAGGGTCCTTCCGCGCCT | ethA | 1,654 |
| ethA-rev | TCAGCGAGCCGTGGCGACCT | ||
| ethB-for | ATGACACTGTCACTGGCCA | ethB | 1,203 |
| ethB-rev | TCACTTCGGGTAGATCCGCA | ||
| ethC-for | ACGGCATCCTCGCCGAGTGC | ethC | 321 |
| ethC-rev | TCAGAACGCGTCGGGGACCT | ||
| ethD-for | ATGTATCAGATCGTGGCCTG | ethD | 312 |
| ethD-rev | CTAGGTCCGGTCGACCTCAT | ||
| tnp-for | ATTCCTGCGGCTACG | L108 IS91-type Y2 tnp | 1,346 |
| tnp-rev | GACACCGTACACATCACA | ||
| mpdJ-2rev_c | CGTCGACGGCAGCCTGCTGG | mpdJ | 380 |
| mpdJ-3for_c | TGTTGTCATCGGTCGGGTGC | ||
| hcmA-c2-for | GACTTCTTCGAGGAGGTCGC | hcmA | 425 |
| hcmA-RPr-2 | GTGCCACCGCGCTTCTCG | ||
| unibac27f | AGAGTTTGATCTGGCTCAG | 16S rRNA gene | 511 |
| univ519r | GTATTACCGCGGCTGCTG | ||
| tnpR-for | TCGTCGCGTGGCAGAGCGCTACCGCGCG | R. ruber IFP 2001 tnpR | 401 |
| tnpR-rev | CGATCACCGCCCTGGCCGACGCGCCGCC | ||
| tnpA-for a | ATGAACACCGTGACGT | R. ruber IFP 2001 tnpA | 810 |
| tnpA-revC | CGCAGTCAACGTCTCCACGC |