Fig. 2.

Iron chelators prevent ferritin induction by thrombin. Cultures (5/condition) were treated with 5 units/ml thrombin for 24 hours alone or with 100 μM deferoxamine (DFO) or 2,2′-bipyridyl (BP). Bars represent mean HO-1 (a) or ferritin (b) band densities, scaled to the mean value in control cultures subjected to sham medium exchange only (= 1). Lane order of representative immunoblots is same as bar order; actin is gel-loading control. *P<0.05, **P<0.01, ***P<0.001 v. sham controls, ##P<0.01 v. thrombin alone condition