Fig 7.

SAL and GA reduce Toxoplasma replication regardless of host cell eIF2α phosphorylation. Tachyzoites were allowed to infect MEFs expressing wild-type (S/S) or nonphosphorylatable (A/A) eIF2α in culture medium supplemented with the indicated concentration of SAL, GA, or vehicle (DMSO). (A) At 24 h postinfection, parasite replication was assessed by counting tachyzoites visualized by IFA through labeling with an Alexa 488-conjugated anti-SAG1 antibody in at least 50 random vacuoles. The graph shows the averages for an experiment performed in triplicate. Error bars indicate standard deviations, and asterisks indicate a statistically significant difference relative to the vehicle control (P < 0.05, two-tailed unpaired t test). (B) At 48 h postinfection, parasite replication was assessed using the PCR-based B1 assay. The histograms show the averages for a representative experiment performed in triplicate. The experiment was repeated three independent times with similar results. Error bars indicate standard deviations, and asterisks indicate a statistically significant difference relative to results for the vehicle control (P < 0.01, two-tailed unpaired t test).