Table 2.
Peptide | % of apoptotic cells |
||||||||
---|---|---|---|---|---|---|---|---|---|
24-h incubation |
48-h incubation |
72-h incubation |
|||||||
2.5 μM | 12.5 μM | 25.0 μM | 2.5 μM | 12.5 μM | 25.0 μM | 2.5 μM | 12.5 μM | 25.0 μM | |
Negative control | 4.2 | 4.2 | 4.2 | 6.6 | 6.6 | 6.6 | 6.3 | 6.3 | 6.3 |
hBD1 | 7.1 | 10.3 | 9.6 | 6.1 | 6.2 | 7 | 8.2 | 7.5 | 4.1 |
hBD3 | 12.6 | 12.8 | 20.8 | 8.7 | 6 | 34.1 | 9.2 | 7.6 | 9.8 |
1C | 9 | 12.3 | 11.7 | 7.8 | 13.6 | 20.6 | 5.2 | 10.9 | 8 |
3I | 6.1 | 6.8 | 7.0 | 5.8 | 6.2 | 7.1 | 5.3 | 6.1 | 7.2 |
3N | 8.2 | 12.3 | 12.9 | 10.8 | 10.3 | 10.1 | 6 | 5.9 | 9.1 |
3NI | 6.6 | 9.2 | 14.3 | 7.5 | 6.2 | 14.2 | 7.3 | 6.3 | 6.2 |
Cells were incubated for 24, 48, or 72 h with all peptides at concentrations of: 2.5, 12.5, and 25.0 μM. Data are expressed as a percentage of apoptotic cells after the subtraction of negative control values (untreated cells). No synthetic peptide significantly increased apoptosis versus the negative control. Experiments were performed in triplicate.