Table 3.
Peptide | % of apoptotic cells |
||||||||
---|---|---|---|---|---|---|---|---|---|
24-h incubation |
48-h incubation |
72-h incubation |
|||||||
2.5 μM | 12.5 μM | 25.0 μM | 2.5 μM | 12.5 μM | 25.0 μM | 2.5 μM | 12.5 μM | 25.0 μM | |
Negative control | 15.2 | 15.2 | 15.2 | 10.2 | 10.2 | 10.2 | 19.6 | 19.6 | 19.6 |
hBD1 | 14.4 | 12.6 | 12.9 | 17.8 | 11.3 | 10.7 | 15.1 | 8.1 | 17.2 |
hBD3 | 14 | 8.3 | 9.2 | 10.9 | 14.8 | 18.7 | 10.8 | 15.9 | 26.2 |
1C | 9.2 | 12.3 | 14 | 16.1 | 16.2 | 16.2 | 21 | 18 | 22 |
3I | 7.0 | 7.3 | 8.1 | 9.2 | 10.3 | 11.4 | 15.2 | 16.8 | 17.6 |
3N | 8.4 | 7.4 | 16.7 | 14.7 | 12.5 | 11.2 | 12.8 | 25.2 | 22.3 |
3NI | 10.7 | 15.5 | 13.1 | 15.4 | 16.9 | 23.4 | 25.6 | 24.5 | 31.8 |
Cells were incubated for 24, 48, and 72 h with all peptides at concentrations of 2.5, 12.5, and 25.0 μM. Data are expressed as a percentage of apoptotic cells after the subtraction of negative control values (untreated cells). No synthetic peptide significantly increased apoptosis versus the negative control. Experiments were performed in triplicate.