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Analysis of the MecI proteolysis by Western blotting. The proteolysis of MecI under induction conditions (sub-MIC of oxacillin at 0.05 mg/liter) was evaluated for prototype strain N315 and recombinant strains overexpressing wild-type and mutant MecI variants [N315(pGC2::mecI) and N315(pGC2::mecIMUT)] using a polyclonal antibody raised against purified MecI.
