Fig 1.

(A) The amount of β-1,3 glucan in a 1-mg/ml sample of purified in vitro biofilm matrix, measured by ELISA. Samples are measured in triplicate. Standard errors are shown. (B) [H³]fluconazole in the matrix, cell wall, and cytoplasm of in vitro biofilms. Shown as a percentage of [H³]fluconazole in the total biofilm, measured as CPM/biofilm. Standard errors are shown. (C) Biofilm susceptibility to fluconazole and/or β-1,3 glucanase as measured by the XTT reduction assay. Fluconazole dosed at 1 mg/ml and β-1,3 glucanase dosed at 0.7 U/ml. Data shown are representative examples, each read in triplicate. *, P < 0.05, comparing the combined drug values to either of the single-drug values using a one-way ANOVA.